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FIGURE 4 Unique transcriptional factors upregulated in RELN positive clusters. (A) Expression of the top twenty enriched transcription factors (TFs, x-axis) were identified for each of the RELN-positive and putative stellate cell (SC) populations (RELN6, 7) and are visualized across the identified progenitor and excitatory neuron populations (y-axis) in the developing medial entorhinal cortex (MEC). The selected genes for reprogramming induced pluripotent stem cells (iPSCs) are highlighted in blue within the RELN7 cluster. (B) The laminar expression of the six stellate cell TFs (Runx1t1, Eya2, Foxp1, <t>Sox5,</t> Tcf4, and Mef2c) was investigated by ISH in sagittal sections of the publicly available Allen Mouse Brain Atlas (Lein et al., 2004; 2007). Image credit: Allen Institute. The red dotted lines demarcate the lamina dissecans.
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FIGURE 4 Unique transcriptional factors upregulated in RELN positive clusters. (A) Expression of the top twenty enriched transcription factors (TFs, x-axis) were identified for each of the RELN-positive and putative stellate cell (SC) populations (RELN6, 7) and are visualized across the identified progenitor and excitatory neuron populations (y-axis) in the developing medial entorhinal cortex (MEC). The selected genes for reprogramming induced pluripotent stem cells (iPSCs) are highlighted in blue within the RELN7 cluster. (B) The laminar expression of the six stellate cell TFs (Runx1t1, Eya2, Foxp1, <t>Sox5,</t> Tcf4, and Mef2c) was investigated by ISH in sagittal sections of the publicly available Allen Mouse Brain Atlas (Lein et al., 2004; 2007). Image credit: Allen Institute. The red dotted lines demarcate the lamina dissecans.
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FIGURE 4 Unique transcriptional factors upregulated in RELN positive clusters. (A) Expression of the top twenty enriched transcription factors (TFs, x-axis) were identified for each of the RELN-positive and putative stellate cell (SC) populations (RELN6, 7) and are visualized across the identified progenitor and excitatory neuron populations (y-axis) in the developing medial entorhinal cortex (MEC). The selected genes for reprogramming induced pluripotent stem cells (iPSCs) are highlighted in blue within the RELN7 cluster. (B) The laminar expression of the six stellate cell TFs (Runx1t1, Eya2, Foxp1, <t>Sox5,</t> Tcf4, and Mef2c) was investigated by ISH in sagittal sections of the publicly available Allen Mouse Brain Atlas (Lein et al., 2004; 2007). Image credit: Allen Institute. The red dotted lines demarcate the lamina dissecans.
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FIGURE 4 Unique transcriptional factors upregulated in RELN positive clusters. (A) Expression of the top twenty enriched transcription factors (TFs, x-axis) were identified for each of the RELN-positive and putative stellate cell (SC) populations (RELN6, 7) and are visualized across the identified progenitor and excitatory neuron populations (y-axis) in the developing medial entorhinal cortex (MEC). The selected genes for reprogramming induced pluripotent stem cells (iPSCs) are highlighted in blue within the RELN7 cluster. (B) The laminar expression of the six stellate cell TFs (Runx1t1, Eya2, Foxp1, <t>Sox5,</t> Tcf4, and Mef2c) was investigated by ISH in sagittal sections of the publicly available Allen Mouse Brain Atlas (Lein et al., 2004; 2007). Image credit: Allen Institute. The red dotted lines demarcate the lamina dissecans.
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FIGURE 4 Unique transcriptional factors upregulated in RELN positive clusters. (A) Expression of the top twenty enriched transcription factors (TFs, x-axis) were identified for each of the RELN-positive and putative stellate cell (SC) populations (RELN6, 7) and are visualized across the identified progenitor and excitatory neuron populations (y-axis) in the developing medial entorhinal cortex (MEC). The selected genes for reprogramming induced pluripotent stem cells (iPSCs) are highlighted in blue within the RELN7 cluster. (B) The laminar expression of the six stellate cell TFs (Runx1t1, Eya2, Foxp1, <t>Sox5,</t> Tcf4, and Mef2c) was investigated by ISH in sagittal sections of the publicly available Allen Mouse Brain Atlas (Lein et al., 2004; 2007). Image credit: Allen Institute. The red dotted lines demarcate the lamina dissecans.
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FIGURE 4 Unique transcriptional factors upregulated in RELN positive clusters. (A) Expression of the top twenty enriched transcription factors (TFs, x-axis) were identified for each of the RELN-positive and putative stellate cell (SC) populations (RELN6, 7) and are visualized across the identified progenitor and excitatory neuron populations (y-axis) in the developing medial entorhinal cortex (MEC). The selected genes for reprogramming induced pluripotent stem cells (iPSCs) are highlighted in blue within the RELN7 cluster. (B) The laminar expression of the six stellate cell TFs (Runx1t1, Eya2, Foxp1, Sox5, Tcf4, and Mef2c) was investigated by ISH in sagittal sections of the publicly available Allen Mouse Brain Atlas (Lein et al., 2004; 2007). Image credit: Allen Institute. The red dotted lines demarcate the lamina dissecans.

Journal: Frontiers in cell and developmental biology

Article Title: Production of human entorhinal stellate cell-like cells by forward programming shows an important role of Foxp1 in reprogramming.

doi: 10.3389/fcell.2022.976549

Figure Lengend Snippet: FIGURE 4 Unique transcriptional factors upregulated in RELN positive clusters. (A) Expression of the top twenty enriched transcription factors (TFs, x-axis) were identified for each of the RELN-positive and putative stellate cell (SC) populations (RELN6, 7) and are visualized across the identified progenitor and excitatory neuron populations (y-axis) in the developing medial entorhinal cortex (MEC). The selected genes for reprogramming induced pluripotent stem cells (iPSCs) are highlighted in blue within the RELN7 cluster. (B) The laminar expression of the six stellate cell TFs (Runx1t1, Eya2, Foxp1, Sox5, Tcf4, and Mef2c) was investigated by ISH in sagittal sections of the publicly available Allen Mouse Brain Atlas (Lein et al., 2004; 2007). Image credit: Allen Institute. The red dotted lines demarcate the lamina dissecans.

Article Snippet: Plasmids containing EYA2 (Addgene #49264), RUNX1T1 (Addgene #49264), TCF4 (Addgene #109144), Foxp1 (Addgene #16362), Sox5 (Addgene #48707) and MEF2C (Addgene #61538) were all acquired from Addgene.

Techniques: Expressing